pGEX-6p1
                                                  BamHI    EcoRI
                                                      |        |
         CATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAATTC
     900 +---------+---------+---------+---------+---------+--------- 959

GST ...  H  P  P  K  S  D  L  E  V  L  F  Q  G\/P  L  G  S  P  E  F   -
                             PreScission site /\

          SalI
       SmaI  | XhoI  NotI
          |  |    |     |
         CCGGGTCGACTCGAGCGGCCGCATCGTGACTGACTGACGAT
     960 +---------+---------+---------+---------+ 1000

         P  G  R  L  E  R  P  H  R  D  *