pQE30NST
      XhoI                            < -35>            < -10>
         CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCA
       1 ---------+---------+---------+---------+---------+---------+ 60
                                EcoRI	         <RBS  >
         ATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACTATGAGA
      61 ---------+---------+---------+---------+---------+---------+ 120
                                                               M  R   
 
                             BamHI     >-------- SP6 ------->SalI
         GGATCGCATCACCATCACCATCACGGATCCtatttaggtgacactatagaatcgtcgacc
     121 ---------+---------+---------+---------+---------+---------+ 180
         G  S  H  H  H  H  H  H  G  S  Y  L  G  D  T  I  E  S  S  T   
              <--- 6xHis tag --> 
                                            
         BglII   NotI      <------ T7 --------<HindIII
         tgcaagatctgcggccgctccctatagtgagtcgtattAAGCTTAATTAGCTGAGCTTGG
     181 ---------+---------+---------+---------+---------+---------+ 240
         C  K  I  C  G  R  S  L  *