pQTEV

EcoRI        <RBS  >
GAATTCATTAAAGAGGAGAAATTAACTATGAAACATCACCATCACCATCACCATAGCGATTACGACATCCCCACTACT
  90        100       110       120       130       140       150       160
                           M  K  H  H  H  H  H  H  H  S  D  Y  D  I  P  T  T
                                 <--- (His)7 tag ----> 

                  BamHI   SalI         BglII  KpnI  NotI                 HindIII
GAGAATCTTTATTTTCAGGGATCCGGGTCGACTGTTGATAGATCTCGGTACCGCGGCCGCTCGACCTGCAGCCAA
    170       180       190       200       210       220       230       240
E  N  L  Y  F  Q\/G  S  G  S  T  V  D  R  S  R  Y  R  G  R  S  T  C  S  Q
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